CBSE Class 12 Biotechnology New Syllabus 2021-22 for Term 1 and Terms 2

Check below full Biotechnology syllabus of CBSE class 12 exam. We have mentioned here CBSE class 12 Biotechnology syllabus 2021 for both written as practical exam. The Biotechnology syllabus of CBSE class 12 exam is divided into two terms- the term I includes Biotechnology: An Overview , Molecules of Life , Genetics and Molecular Biology and term II includes Genetics and Molecular Biology (Contd.), Cells and Organisms and Practical.

CBSE Class 12 Biotechnology New Syllabus 2021

CBSE class 12 new syllabus for Biotechnology is provided below-

Units  Term-I Marks 
Unit-I Biotechnology: An Overview 5
Unit-II Molecules of Life 20
Unit-III Genetics and Molecular Biology 10
Term-II
Unit-III Genetics and Molecular Biology (Contd.) 10
Unit-IV Cells and Organisms 25
Practical 30
Total 100

TERM-I (35 Marks)

Unit-V Protein and Gene Manipulation

Chapter-1: Recombinant DNA Technology

Introduction, Tool of DNA technology, Making of rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter-2: Protein Structure and Engineering 

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics 

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics,

TERM-II

Unit-V Protein and Gene Manipulation 

Information sources, Analysis using bioinformatics tools.                                                              05 Marks

Unit-VI Cell Culture and Genetic Manipulation                                                                              30 Marks

Chapter-1: Microbial Cell Culture and its Applications 

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications 

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture,

Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications 

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

PRACTICAL

Term I

Practical should be conducted alongside the concept taught in theory classes 

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Reading of a DNA sequencing gel to arrive at the sequence
  6. Project Work

Note:- More emphasis should be given on hands on working projects.

The scheme of evaluation at the end of term will be as under:

A One experiments 06
  Practical record 02
    Viva on Practical 02
B Project Work 05
Total 15

Term II

  1. Isolation of bacteria from curd & staining of bacteria
  2. Cell viability assay using Evan’s blue dye exclusion method
  3. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
  4. Project Work

The scheme of evaluation at the end of term will be as under:

A One experiments 06
                          Practical record 02
Viva on Practical 02
B Project work 05
            Total 15

Note:- More emphasis should be given on hands on work in projects.

Prescribed Books: 

A Text Book of Biotechnology – Class XI : Published by CBSE, New Delhi

As reference- Biotechnology – Class XI : Published by NCERT, New Delhi

A Laboratory Manual of Biotechnology – Class XI : Published by CBSE, New Delhi

A Text Book of Biotechnology – Class XII : Published by CBSE, New Delhi

A Laboratory Manual of Biotechnology – Class XII : Published by CBSE, New Delhi

CheckCBSE class 12 Biotechnology syllabus pdf

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